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KMID : 0545120080180040704
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Journal of Microbiology and Biotechnology
2008 Volume.18 No. 4 p.704 ~ p.710
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Effect of Increased Glutamate Availability on L-Ornithine Production in Corynebacterium glutamicum
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Hwang Joong-Hee
Hwang Gui-Hye
Cho Jae-Yong
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Abstract
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Glutamate availability in the argF-argR-proBΔ strain of Corynebacterium glutamicum was increased by addition of glutamate to the cell or inactivation of the phosphoenolpyruvate carboxykinase activity and simultaneous overexpression of the pyruvate carboxylase activity to assess its effect on Lornithine production. When glutamate was increased in an Lornithine- producing strain, the production of L-ornithine was not changed. This unexpected result indicated that the intracellular concentration and supply of glutamate is not a rate-limiting step for the L-ornithine production in an L-ornithine-producing strain of C. glutamicum. In contrast, overexpression of the L-ornithine biosynthesis genes (argCJBD) resulted in approximately 30% increase of L-ornithine production, from 12.73 to 16.49 mg/g (dry cell weight). These results implied that downstream reactions converting glutamate to L-ornithine, but not the availability of glutamate, is the rate-limiting step for elevating L-ornithine production in the argF-argR-proBΔ strain of C. glutamicum.
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KEYWORD
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argCJBD, Corynebacterium glutamicum, glutamate availability, L-ornithine production, rate-limiting
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